ABSTRACT
Las pruebas fisiológicas y bioquímicas constituyen dos de las principales metodologías utilizadas, principalmente en los laboratorios de microbiología, para la identificación y diferenciación de los actinomicetos. La finalidad de este trabajo fue comparar y evaluar los métodos fenotípicos que son utilizados de manera rutinaria en la identificación de estos microorganismos. Se estudiaron setenta y tres cepas de actinomicetos provenientes de tres laboratorios de microbiología de Venezuela. El comportamiento fisiológico y bioquímico de las cepas en estudio fue evaluado mediante pruebas de descomposición de diferentes sustratos. Los resultados obtenidos permitieron observar diferencias en la identificación preliminar de las cepas realizada por estos laboratorios, conduciendo algunas veces a una nueva identificación de las mismas, gracias a la utilización de estos sustratos. Se pudo observar que la metodología empleada permitió la reclasificación taxonómica de casi todas las cepas estudiadas, lo que sugiere que es necesario estandarizar la metodología de identificación para los actinomicetos.
Physiologic and biochemical tests constitute two of the main methodologies, mainly used in microbiology laboratories, for the identification and differentiation of actinomyces. The purpose of this work was to compare and evaluate the phenotypic methods used routinely for the identification of these microorganisms. The study included seventy three actinomyces strains from three microbiology laboratories in Venezuela. The physiologic and biochemical behavior of the strains was evaluated through decomposition tests using different substrates. The results obtained showed differences in the preliminary identification of the strains done in the various laboratories, leading occasionally to a new identification arisen from the use of these substrates. It was seen that with the methods used it was possible to taxonomically reclassify almost all the strains studied, suggesting that it is necessary to standardize the methods for the identification of actinomyces.
ABSTRACT
Las bacterias del género Nocardia son actinomicetos aerobios cuyo hábitat es el suelo y la materia orgánica en descomposición. La infección natural por especies de este género ha sido reportada en humanos y animales, sin embargo, la infección natural en ratones no ha sido descrita. En este estudio se demuestra por primera vez el aislamiento de la especie recientemente descrita, Nocardia cyriacigeorgica, en pulmones de ratones Balb/c. El estudio microbiológico de las muestras de pulmón reportó la presencia de filamentos grampositivos ramificados fragmentados en forma cocobacilares. Los estudios de PCR demostraron que la especie aislada fue N. cyriacigeorgica. Se evidencia en este trabajo que N. cyriacigeorgica es potencialmente patógena en ratones Balb/c.
Bacteria belonging to the Nocardia genus are aerobic actinomycetae whose habitat is the ground and decomposing organic material. Natural infection by species belonging to this genus has been reported in humans and animals; nevertheless, natural infection in mice has not been described until now. This study demonstrates for the first time the isolation of a recently described species, Nocardia cyriacigeorgica, rom the lungs of Balb/c mice. The microbiological study of the lung samples reported the presence of branched Gram positive filaments with a cocobacillar shape. PCR studies demonstrated that the species isolated was N. cyriacigeorgica. This study demonstrates that N. cyriacigeorgica is potentially pathogenic for Balb/c mice.
ABSTRACT
We propose to apply a new sub model of the case-control-community-based epidemiologic design as a model to biomedical and epidemiologic research aimed to develop advanced and holistic research on human deep or localized mycoses transmitted from environment-to-man, in addition to classic or empirical epidemiologies, which by them selves are suitable to develop research on cutaneous, nail, or ocular mycoses. Our own contribution to complement and enhance research on deep and localized mycoses is the multidisciplinary family-case biomedical and epidemiologic model very useful to carry out biomedical and epidemiologic research on pathogenic deep and localized mycoses infections or diseases in man. In fact, the multidisciplinary family-case epidemiologic approach would also be a powerful for comprehensive research studies on chronic infections and syndromes asociated to paracoccidioidomycosis, chromomycosis, coccidioidomycosis, sporotrichosis, eumycetoma and actinomycetoma. The common starting point of such to be follewed in order to study any of those mycoses is based on a nested group of cases which have been previously well-defined as clinical deep mycotic patients from any of the research groups in Latin American countries. African countries, India, etc. Two control groups will allow to make best inferences: a) relatives face of clinical disease living in the same household of each cases (group # 1); b) adults living in other neighbor household where clinical cases of the specific deep mycoses studied has never appeared. In successful, results of application of this model would be established as standards to estimate prevalence and incidence of environment-to-man transmitted infections. That would means, potential application to reach primary and secondary prevention to improve local or regional public health, at least in South America